HX Protocol Week 6 Culture

Single cell transcriptomes of cell cultures differentiated from human pluripotent stem cells (hPSCs) following the HX protocol (Xi et al., Cell Rep, 2017) for 6 weeks. Cells are either non-enriched or enriched using an endogenous PAX7-driven GFP reporter.

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HX Protocol Week 5 Myogenic Subset

Single cell transcriptomes of the skeletal muscle (SkM) lineage from human pluripotent stem cell (hPSCs) differentiated for 5 weeks using the HX protocol (Xi et al., Cell Rep, 2017). Cells are subjected to re-clustering to reveal potential heterogeneity within the myogenic subset.

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HX Protocol Week 5 Culture

Single cell transcriptomes of cell cultures differentiated from human pluripotent stem cells (hPSCs) following the HX protocol (Xi et al., Cell Rep, 2017) for 5 weeks. Cells are either non-enriched, or enriched using an endogenous PAX7-driven GFP reporter or cell surface marker combination of ERBB3+NGFR+HNK1-.

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HX Protocol Week 4 Myogenic Subset

Single cell transcriptomes of the skeletal muscle (SkM) lineage from human pluripotent stem cell (hPSCs) differentiated for 4 weeks using the HX protocol (Xi et al., Cell Rep, 2017). Cells are subjected to re-clustering to reveal potential heterogeneity within the myogenic subset.

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HX Protocol Week 4 Culture

Single cell transcriptomes of cell cultures differentiated from human pluripotent stem cells (hPSCs) following the HX protocol (Xi et al., Cell Rep, 2017) for 4 weeks. Cells are either non-enriched or enriched using an endogenous PAX7-driven GFP reporter.

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HX Protocol Week 3 Culture

Single cell transcriptomes of cell cultures differentiated from human pluripotent stem cells (hPSCs) following the HX protocol (Xi et al., Cell Rep, 2017) for 3 weeks. Cells are either non-enriched or enriched using an endogenous PAX7-driven GFP reporter.

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Adult Myogenic Subset

Single cell transcriptomes of the skeletal muscle (SkM) lineage from the quadriceps muscles of human adults. Cells are subjected to re-clustering to reveal potential heterogeneity within the myogenic subset. Two independent biological samples at 34 and 42 years old are included.

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Adult Hindlimb Muscle

Single cell transcriptomes of the quadriceps muscles from human adults. Hematopoietic and endothelial cells are pre-excluded by FACS. Two independent biological samples at 34 and 42 years old are included.

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Fetal Week 12-14 Myogenic Subset

Single cell transcriptomes of the skeletal muscle (SkM) lineage from hindlimbs of 12-14 week human fetuses. Cells are subjected to re-clustering to reveal potential heterogeneity within the myogenic subset. Two independent biological samples are included.

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Fetal Week 12-14 Hindlimb Muscle

Single cell transcriptomes of the total skeletal muscle tissues of hindlimbs from human fetuses of 12-14 weeks of development. Hematopoietic and endothelial cells are pre-excluded by FACS. Two independent biological samples are included.

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