Publications

2020

Xi H, Langerman J, Sabri S, Chien P, Young CS, Hicks M, Gonzalez K, Fujiwara W, Marzi J, Liebscher S, Spencer M, Van Handel B, Evseenko D, Schenke-Layland K, Plath K, Pyle AD.  A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Cell Stem Cell, May 11, 2020.PMID:32396864

2019

Mosich GM, Husman R, Shah P, Sharma A, Rezzadeh K, Aderibigbe T, Hu VJ, McClintick DJ, Wu G, Gatto JD, Xi H, Pyle AD, Péault B, Petrigliano FA, Dar A.Non-fibro-adipogenic pericytes from human embryonic stem cells attenuate degeneration of the chronically injured mouse muscle.JCI Insight. 2019 Dec 19;4(24). pii: 125334. doi: 10.1172/jci.insight.125334.
PMID:31852842

Xi H, Pyle A. Beyond the genome: RNA control of stem cells.
Science. 2019 Nov 8;366(6466):684-685. doi: 10.1126/science.aaz4859. No abstract available.
PMID:31699921

Young CS, Pyle AD, Spencer MJ. CRISPR for Neuromuscular Disorders: Gene Editing and Beyond.Physiology (Bethesda). 2019 Sep 1;34(5):341-353. doi: 10.1152/physiol.00012.2019. Review.
PMID:31389773

Bellmann J, Goswami RY, Girardo S, Rein N, Hosseinzadeh Z, Hicks MR, Busskamp V, Pyle AD, Werner C, Sterneckert J. A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits. Biomaterials. 2019 Dec;225:119537.

Emami ME#, Young CS#, Ji Y#, Liu X, Mokhonova E, Pyle AD*, Meng H*, Spencer MJ*. Polyrotaxane Nanocarriers Can Deliver CRISPR/Cas9 Plasmid to Dystrophic Muscle Cells to Successfully Edit the DMD Gene. Advanced Therapeutics. June 3, 2019. # co-first, *co-corresponding

2018

Hicks MR, Hiserodt J, Paras K, Fujiwara W, Eskin A, Jan M, Xi H, Young C, Evseenko D, Nelson S, Spencer M, Van Handel B, Pyle AD.  ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs.  Nature Cell Biology, 20, 46–57. Jan 1, 2018.

Ferguson GB, Van Handel B, Bay M, Fiziev P, Org T, Lee S, Shkhyan R, Banks NW, Scheinberg M, Wu L, Saitta B, Elphingstone J, Larson AN, Riester SM, Pyle AD, Bernthal NM, Mikkola HK, Ernst J, van Wijnen AJ, Bonaguidi M, Evseenko D. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nat Commun. 2018 Sep 7;9(1):3634.

2017

Young CS, Mokhonova E, Quinonez M, Pyle AD, Spencer MJ. Creation of a Novel Humanized Dystrophic Mouse Model of Duchenne Muscular Dystrophy and Application of a CRISPR/Cas9 Gene Editing TherapyJ Neuromuscul Dis, 2017; 4(2):139-145.

Hazima RA, Karumbayaramb S, Dimashkiee A, Jianga M, Lopesa VS, Lia D, Burgessa BL, Vijayara P, Alva-Ornelas JA, Zack JA, Kohn DB, Gomperts BN, Pyle AD, Lowry WE, Williams D.  Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Research & Therapy, 8:217, 2017.

Xi H, Fujiwara W, Gonzalez K, Jan M, Liebscher S, Van Handel B, Schenke-Layland K, Pyle AD.  In Vivo Human Somitogenesis Guides Somite Development from hPSCs.  Cell Reports.  18, 1-13. Feb 7, 2017.

2016

Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ*, Pyle AD*. A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells, Cell Stem Cell, 2016; 18: 1-8. * co-corresponding

Lee PC, Truong B, Vega-Crespo A, Gilmore WB, Hermann K, Angarita SA, Tang JK, Chang KM, Wininger AE, Lam AK, Schoenberg BE, Cederbaum SD, Pyle AD, Byrne JA, Lipshutz GS.  Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells.  Mol Ther Nucleic Acids. 2016 Nov 29;5(11).

Young CS, Pyle AD.  Exon Skipping Therapy.  Cell. 2016 Nov 17;167(5):1144.

2015

Thakore-Shah K, Koleilat T, Jan M, John A, Pyle AD. REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells, Plos One, 2015; 10(12). (link)

Hicks M, Pyle A. The Path from Pluripotency to Skeletal Muscle: Developmental Myogenesis Guides the Way, Cell Stem Cell, 2015; 17(3): 255-7. (link)

2014

Peng J, Garcia MA, Choi J, Zhao L, Chen K, Bernstein JR, Peyda P, Hsiao Y, Liu KW, Lin W, Pyle AD, Wang H, Hou S, Tseng H. Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency, ACS nano, 2014; 8(5): 4621-9. (link)

2013

Awe JP, Lee PC, Ramathal C, Vega-Crespo A, Durruthy-Durruthy J, Cooper A, Karumbayaram S, Lowry WE, Clark AT, Zack JA, Sebastiano V, Kohn DB, Pyle AD, Martin MG, Lipshutz GS, Phelps PE, Pera RA, Byrne JA. Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status, Stem cell research & therapy, 2013; 4(4): 87. (link)

Yanes RE, Tarn D, Hwang AA, Ferris DP, Sherman SP, Thomas CR, Lu J, Pyle AD, Zink JI, Tamanoi F. Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition Small (Weinheim an der Bergstrasse, Germany), 2013; 9(5): 697-704. (link)

Palomares K, Vigant F, Van Handel B, Pernet O, Chikere K, Hong P, Sherman SP, Patterson M, An Dong S, Lowry WE, Mikkola HK, Morizono K, Pyle AD, Lee B. Nipah virus envelope-pseudotyped lentiviruses efficiently target ephrinB2-positive stem cell populations in vitro and bypass the liver sink when administered in vivo, Journal of virology, 2013; 87(4): 2094-108. (link)

Sherman SP, Pyle AD. Small molecule screening with laser cytometry can be used to identify pro-survival molecules in human embryonic stem cells, PloS one, 2013; 8(1): e54948. (link)

2012

Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE. From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols Stem cells translational medicine, 2012; 1(1): 36-43. (link)

2011

Alva JA, Lee GE, Escobar EE, Pyle AD. Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells, Stem cells (Dayton, Ohio), 2011; 29(12): 1952-62. (link)

Sherman SP, Alva JA, Thakore-Shah K, Pyle AD. Human pluripotent stem cells: the development of high-content screening strategies Methods in molecular biology (Clifton, N.J.), 2011; 767(1): 283-95. (link)

Le Belle JE, Orozco NM, Paucar AA, Saxe JP, Mottahedeh J, Pyle AD, Wu H, Kornblum HI. Proliferative neural stem cells have high endogenous ROS levels that regulate self-renewal and neurogenesis in a PI3K/Akt-dependent manner Cell stem cell, 2011; 8(1): 59-71. (link)

2010

Tchieu J, Kuoy E, Chin MH, Trinh H, Patterson M, Sherman SP, Aimiuwu O, Lindgren A, Hakimian S, Zack JA, Clark AT, Pyle AD, Lowry WE, Plath K. Female human iPSCs retain an inactive X chromosome Cell stem cell, 2010; 7(3): 329-42. (link)

Tseng H, Arasteh A, Paranjpe A, Teruel A, Yang W, Behel A, Alva JA, Walter G, Head C, Ishikawa T, Herschman HR, Cacalano N, Pyle AD, Park N, Jewett A. Increased lysis of stem cells but not their differentiated cells by natural killer cells; de-differentiation or reprogramming activates NK cells, PloS one, 2010; 5(7): e11590. (link)

Kamei K, Ohashi M, Gschweng E, Ho Q, Suh J, Tang J, For Yu ZT, Clark AT, Pyle AD, Teitell MA, Lee K, Witte ON, Tseng H. Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions, Lab on a chip, 2010; 10(9): 1113-9. (link)

2009

Chin MH, Mason MJ, Xie W, Volinia S, Singer M, Peterson C, Ambartsumyan G, Aimiuwu O, Richter L, Zhang J, Khvorostov I, Ott V, Grunstein M, Lavon N, Benvenisty N, Croce CM, Clark AT, Baxter T, Pyle AD, Teitell MA, Pellegrini M, Plath K, Lowry WE. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures, Cell stem cell, 2009; 5(1): 111-23. (link)

Zeng W, de Greef JC, Chen Y, Chien R, Kong X, Gregson HC, Winokur ST, Pyle A, Robertson KD, Schmiesing JA, Kimonis VE, Balog J, Frants RR, Ball AR, Lock LF, Donovan PJ, van der Maarel SM, Yokomori K. Specific loss of histone H3 lysine 9 trimethylation and HP1gamma/cohesin binding at D4Z4 repeats is associated with facioscapulohumeral dystrophy (FSHD), PLoS genetics, 2009; 5(7): e1000559. (link)

Damoiseaux R, Sherman SP, Alva JA, Peterson C, Pyle AD. Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells, Stem cells (Dayton, Ohio), 2009; 27(3): 533-42. (link)

Conway AE, Lindgren A, Galic Z, Pyle AD, Wu H, Zack JA, Pelligrini M, Teitell M, Clark AT. A self-renewal program controls the expansion of genetically unstable cancer stem cells in pluripotent stem cell-derived tumors, Stem cells (Dayton, Ohio), 2009; 27(1): 18-28. (link)

2008

Wu H, Kim KJ, Mehta K, Paxia S, Sundstrom A, Anantharaman T, Kuraishy AI, Doan T, Ghosh J, Pyle AD, Clark A, Lowry W, Fan G, Baxter T, Mishra B, Sun Y, Teitell MA. Copy number variant analysis of human embryonic stem cells, Stem cells (Dayton, Ohio), 2008; 26(6): 1484-9. (link)